Publications
Our publications span analytical platform development (CE–MS and complementary MS), software and data standards, and application-driven glyco(proteo)mics—with a strong emphasis on biomarker-oriented oncology research. For a curated narrative of our application work (including PSA, PAP, and CEA highlights), see the Applications page; the sections below group publications by theme to help you quickly find work relevant to your question or sample type.
This section highlights our most recent manuscripts (preprints/accepted/in press) alongside field-shaping reviews, reporting guidelines, and software outputs. It provides a quick entry point to what’s new, what we contribute to community standards, and how we make glyco(proteo)mics analysis more scalable.
General
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Dessidianti, R., Grabarics, M., Virág, D., Lageveen-Kammeijer, G.S.M., Szökő, É., Turiák, L. Capillary electrophoresis in glycosaminoglycan analysis.Talanta 2026, 303, 129429. DOI: 10.1016/j.talanta.2026.129429
Wang, W., Kuzyk, V., Lageveen-Kammeijer, G.S.M., Palmblad, M. Semantic Annotation of Glycomics and Glycoproteomics Methods. Glycobiology 2025. (Just accepted). DOI: 10.1093/glycob/cwaf072
Tol, B.D.M., van, Doodewaerd, B.R., van, Lageveen-Kammeijer, G.S.M., Jansen, B.C., Liwocha, J., Mukhopadhyay, R., Wuhrer, M., Heden van Noort, G.J. van der, Schulman, B.A., Geurink, P.P. Employing neutron-encoded monoUbs to study E2/E3 ligase activity and selectivity for assembling Ub chains. bioRxiv 2025. DOI: 10.1101/2025.05.22.655449 (Preprint)
Xiao, R., Zheng, J., Lo Ponte, H.F.B.R., Ding, Y., Gross-Valle, C., Derks, T.G.J., Horvatovich, P.L., Heiner-Fokkema, M.R., Bakker, B.M., Wolters, J.C.*, Lageveen-Kammeijer, G.S.M. * Decoding the Glycan Signature: Unraveling N-Glycosylation Alterations in Glycogen Storage Disease Ia and Ib. bioRxiv submission 2025-10-23 (MS ID#: BIORXIV/2025/684118). (Preprint)
Loponte, H.F., Zheng, J., Ding, Y., Oliveira, I.A., Basse, K., Todeschini, A.R.,* Horvatovich, P.L.,* Lageveen-Kammeijer, G.S.M. * GlycoGenius: the ultimate high-throughput glycan composition identification tool. Nature Communications 2025, 16(1), 10335. DOI: s41467-025-65265-2
Guo, R-R, Heijs, B., Wang, W., Wuhrer, M., Liu, L., Lageveen-Kammeijer, G.S.M., Voglmeir, J.,Insight into distribution and composition of nonhuman N-Glycans in mammalian organs via MALDI-TOF and MALDI-MSI. Carbohydrate Polymers 2025, 351, 123065. DOI: 10.1016/j.carbpol.2024.123065
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Trbojević-Akmačić, I., Lageveen-Kammeijer, G.S.M., Heijs, B., Petrović, T., Deriš, H., Wuhrer, M., Lauc, G. High-throughput glycomic methods. Chemical Reviews 2022, 122(20), 15865–15913. DOI: 10.1021/acs.chemrev.1c01031
Lageveen-Kammeijer, G.S.M., Rapp, E., Chang, D., Rudd, P.M., Kettner, C., Zaia, J. The minimum information required for a glycomics experiment (MIRAGE): reporting guidelines for capillary electrophoresis. Glycobiology 2022, 32(7). DOI: 10.1093/glycob/cwac021
Lageveen-Kammeijer, G.S.M., Kuster, B., Reusch, D., Wuhrer, M. High Sensitivity Glycomics in Biomedicine. Mass Spectrometry Reviews 2021, 41(6), 1014–1039. DOI: 10.1002/mas.21730
Leoz, de M.L.A., … Haan, de N., Falck, D., Lageveen-Kammeijer, G.S.M., Wuhrer, M., … Stein, S.E. NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Analytical Methods. Molecular and Cellular Proteomics 2020, 19(1), 11–30. DOI: 10.1074/mcp.RA119.001677 (author list abbreviated in source list)
Clerc, F., Reiding, K.R., Jansen, B.C., Lageveen-Kammeijer, G.S.M., Bondt, A., Wuhrer, M. Human Plasma Protein N-Glycosylation.Glycoconjugate Journal 2016, 33(3), 309–343. DOI: 10.1007/s10719-015-9626-2
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Loponte, H.F., Zheng, J., Ding, Y., Oliveira, I.A., Basse, K., Todeschini, A.R.,* Horvatovich, P.L.,* Lageveen-Kammeijer, G.S.M. * GlycoGenius: the ultimate high-throughput glycan composition identification tool. Nature Communications 2025, 16(1), 10335. DOI: s41467-025-65265-2
Wang, W., Kuzyk, V., Lageveen-Kammeijer, G.S.M., Palmblad, M. Semantic Annotation of Glycomics and Glycoproteomics Methods. Glycobiology 2025. (Just accepted). DOI: 10.1093/glycob/cwaf072
Moran, A.B., Domínguez-Vega, E., Wuhrer, M., Lageveen-Kammeijer, G.S.M. Software-Assisted Data Processing Workflow for Intact Glycoprotein Mass Spectrometry. Journal of Proteome Research 2023, 22(4), 1367–1376. DOI: 10.1021/acs.jproteome.2c00762
Here we present analytical and workflow innovations that improve sensitivity, specificity, and structural resolution in glyco(proteo)mics, with a strong focus on CE–MS and complementary MS strategies. These papers form the technical foundation for our application-driven studies and assay development.
Method Development
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Lageveen-Kammeijer, G.S.M., Haan, de N., Mohaupt, P., Wagt, A.P., Filius, M., Nouta, J., Falck, D., Wuhrer, M. Highly Sensitive CE-ESI-MS Analysis of N-Glycans from Complex Biological Samples. Nature Communications (2019) 10, 2137
Drouin, N., … Lageveen-Kammeijer, G.S.M., Nouta, J., … Ramautar, R. Capillary electrophoresis-Mass spectrometry at Trial by Metabo-ring: Effective electrophoretic mobility for Reproducible and Robust Compound Annotation. Analytical Chemistry (2020) 92(20), 14103–14112. DOI: 10.1021/acs.analchem.0c03129
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Wagt, S., Haan, N. de, Wang, W., Zhang, T., Wuhrer, M. Lageveen-Kammeijer, G.S.M. N-Glycan Isomer Differentiation by Zero Flow Capillary Electrophoresis Coupled to Mass Spectrometry. Analytical Chemistry (2022) 94(38), 12954–12959. DOI: 10.1021/acs.analchem.2c02840
Moran, A.B., Gardner, R.A., Wuhrer, M., Lageveen-Kammeijer, G.S.M., Spencer, D.I.R. Sialic acid derivatization of fluorescently labeled N-glycans allows linkage differentiation by RPLC-FD-MS. Analytical Chemistry (2022) 94(18). DOI: 10.1021/acs.analchem.1c02610
Kammeijer, G.S.M., Kohler, I., Jansen, B.C., Heemskerk, A.A.M., Mayboroda, O.A., Hensbergen, P.J., Schappler, J., Wuhrer, M. Sialic Acid Linkage Differentiation of Glycopeptides using Capillary Electrophoresis – Electrospray Ionization – Mass Spectrometry.Scientific Reports (2017) 7, 3733. DOI: 10.1038/s41598-017-03838-y
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Madunić, K., Wagt, S., Zhang, T., Wuhrer, M., Lageveen-Kammeijer, G.S.M. Dopant-enriched nitrogen gas for enhanced electrospray ionization of released glycans in negative ion mode. Analytical Chemistry (2021) 93(18), 6919–6923. DOI: 10.1021/acs.analchem.1c00023
Kammeijer, G.S.M., Kohler, I., Jansen, B.C., Hensbergen, P.J., Mayboroda, O.A., Falck, D. Wuhrer, M. Dopant Enriched Nitrogen Gas Combined with Sheathless Capillary Electrophoresis – Electrospray Ionization - Mass Spectrometry for Improved Sensitivity and Repeatability in Glycopeptide Analysis. Analytical Chemistry (2016) 88(11), 5849–5856. DOI: 10.1021/acs.analchem.6b00479
Rebello, O.D., Nicolardi, S., Lageveen-Kammeijer, G.S.M., Nouta, J., Gardner, R.A., Mesker, W.A., Tollenaar, R.A.E.M., Spencer, D.I.R., Wuhrer, M., Falck, D. A matrix-assisted laser desorption/ionization – mass spectrometry assay for the relative quantitation of antennary fucosylated N-glycans in human plasma. Frontiers in Chemistry (2020) 8, 138. DOI: 10.3389/fchem.2020.00138
Bondt, A., Nicolardi, S., Jansen, B.C., Stavenhagen, K., Blank, D., Kammeijer, G.S.M., Kozak, R.P., Fernandes, D.L., Hensbergen, P.J., Hazes, J.M.W., van der Burgt, Y.E.M., Dolhain, R.J.E.M., Wuhrer, M. Longitudinal Monitoring of Immunoglobulin A Glycosylation During Pregnancy by Simultaneous MALDI-FTICR-MS Analysis of N- and O-Glycopeptides. Scientific Reports (2016) 6, 27955. DOI: 10.1038/srep23296
Jansen, B.C., Bondt, A., Reiding, L., Lonardi, E., Jong, de C.J., Falck, D., Kammeijer, G.S.M., Dolhain, R.J.E.M., Rombouts, Y., Wuhrer, M. Pregnancy-Associated Serum N-Glycome Changes Studied by High-Throughput MALDI-TOF-MS. Scientific Reports (2016) 6, 23296. DOI: 10.1038/srep23296
Cross-listed (see also Cancer Biomarkers):
Kammeijer, G.S.M. Analytical Chemistry (2018) — PSA urinary assay (method + application)
Pont, L. JPR (2021) — CEA CE–MS/MS (method + application)
This section collects our biomarker-oriented oncology work, spanning protein-of-interest assays (PSA, PAP, CEA/CEACAM5), biofluid and cell-model studies, and cancer-relevant glycosylation signatures. The emphasis is on translating glycosylation and proteoform information into interpretable readouts for detection, stratification, and monitoring.
Cancer Biomarkers & Oncology Applications
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Wang, W., Nier, C.R. de, Wuhrer, M., Lageveen-Kammeijer, G.S.M. In-Depth Glycoproteomic Assay of Urinary Prostatic Acid Phosphatase. ACS Measurement Sciences AU (2024) 4(1), 117–126. DOI: 10.1021/acsmeasuresciau.3c00055
Wang, W., Zhang, T., Nouta, J., Veelen, P.A. van, Haan, N. de, Reijke, T.M. de, Wuhrer, M., Lageveen-Kammeijer, G.S.M. Human Prostate-Specific Antigen Carries N-glycans with Ketodeoxynononic Acid. Engineering (2023) 26, 119–131. DOI: 10.1016/j.eng.2023.02.009
Moran, A.B., Dominguez-Vega, E., Nouta, J., Pongracz, T., Reijke, T.M. de, Wuhrer, M., Lageveen-Kammeijer, G.S.M. Profiling the proteoforms of urinary prostate-specific antigen by capillary electrophoresis–mass spectrometry. Journal of Proteomics (2021) 238, 104148. DOI: 10.1016/j.prot.2021.104148
Wang, W., Kałuża, A., Nouta, J., Nicolardi, S., Ferens-Sieczkowska, M., Wuhrer, M., Lageveen-Kammeijer, G.S.M.*, Haan, N. de*, High-throughput glycopeptide profiling of prostate-specific antigen from seminal plasma by MALDI-MS. Talanta (2021) 222, 121495. DOI: 10.1016/j.talanta.2020.121495
Kammeijer, G.S.M., Nouta, J., Rosette, de la J.J.M.C.H, Reijke, de T.M., Wuhrer, M. An In-depth Glycosylation Assay for Urinary Prostate-Specific Antigen.Analytical Chemistry (2018) 90(7), 4414–4421. DOI: 10.1021/acs.analchem.7b04281
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Moran, A.B., Elgood-Hunt, G., Burgt, Y.E.M. van der, Wuhrer, M., Mesker, W.E., Tollenaar, R.A.E.M., Spencer, D.I.R., Lageveen-Kammeijer, G.S.M. Serum N-Glycosylation RPLC-FD-MS Assay to Assess Colorectal Cancer Surgical Interventions. Biomolecules (2023) 13(6), 896. DOI: 10.3390/biom13060896
Wang, D., Kuzyk, V., Madunić, K., Zhang, T., Mayboroda, O.A., Wuhrer, M., Lageveen-Kammeijer, G.S.M. In-Depth Analysis of the N-Glycome of Colorectal Cancer Cell Lines. International Journal of Molecular Sciences (2023) 24(5), 4842. DOI: 10.3390/ijms24054842
Madunić, K., Luijkx, Y.M.C.A., Mayboroda, O.A., Janssen, G.M.C., Veelen, P.A. van, Strijbis, K., Wennetkes, T., Lageveen-Kammeijer, G.S.M., Wuhrer, M. O-glycomic and proteomic signatures of spontaneous and butyrate-stimulated colorectal cancer cell line differentiation. Molecular and Cellular Proteomics (2023) 22(3). DOI: 10.1016/j.mcpro.2023.100501
Wang, D., Madunić, K., Zhang, T, Mayboroda, O.A., Lageveen-Kammeijer, G.S.M., Wuhrer, M. High diversity of glycosphingolipid glycans of colorectal cancer cell lines reflects the cellular differentiation phenotype. Molecular and Cellular Proteomics (2022) 21(6). DOI: 10.1016/j.mcpro.2022.100239
Pont, L., Kuzyk, V., Benavente, F., Sanz-Nebot, V., Mayboroda, O.A., Wuhrer, M., Lageveen-Kammeijer, G.S.M. Site-Specific N-Linked Glycosylation Analysis of Human Carcinoembryonic Antigen by Sheathless Capillary Electrophoresis-Tandem Mass Spectrometry. Journal of Proteome Research (2021) 20(3), 1666–1675. DOI: 10.1021/acs.jproteome.0c00875
Madunić, K., Zhang, T., Mayboroda, O.A., Holst, S., Stavenhagen, K., Jin, C., Karlsson, N., Lageveen-Kammeijer, G.S.M., Wuhrer, M. Colorectal cancer cell lines show striking diversity of their O-glycome. Cellular and Molecular Life Sciences (2020) 78(1), 337–350. DOI: 10.1007/s00018-020-03504-z
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Blöchl, C., Wang, D., Mayboroda, O.A., Lageveen-Kammeijer, G.S.M., Wuhrer, M. Transcriptionally imprinted glycomic signatures of acute myeloid leukemia. Cell & Bioscience (2023) 13(1), 31. DOI: 10.1186/s13578-023-00981-0
Wang, D., Zhang, T, Madunić, K., de Waard, A.A., Blöchl, C., Mayboroda, O.A., Griffioen, M., Spaapen, R.M., Huber, C.G., Lageveen-Kammeijer, G.S.M., Wuhrer, M. Glycosphingolipid-Glycan Signatures of Acute Myeloid Leukemia Cell Lines Reflect Hematopoietic Differentiation. Journal of Proteome Research (2022) 21(4), 1029–1040. DOI: 10.1021/acs.jproteome.1c00911
Blöchl, C., Wang, D., Madunic, K., Lageveen-Kammeijer, G.S.M., Huber, C.G., Wuhrer, M., Zhang. Integrated N- and O-Glycomics of Acute Myeloid Leukemia (AML) Cell Lines. Cells (2021) 10(11), 3058. DOI: 10.3390/cells10113058
Cross-listed (see also Tissue & Spatial, and Multi-omics Integration):
Wang, D. … Engineering (2022) — Microdissected CRC regions
Wang, D. … MCP (2024) — (sialyl) Lewis across GSL/N/O- glycans linked to differentiation
These publications focus on glycosylation in immune-mediated and inflammatory contexts, including systemic signatures and immunoglobulin glycosylation. Together, they illustrate how glycan remodeling tracks immune state and can support biomarker discovery beyond oncology.
Immunology, Inflammation & Systemic Disease
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Mayboroda, O.A., Lageveen-Kammeijer, G.S.M., Wuhrer, M., Dolhain, R.J.E.M., An Integrated Glycosylation Signature of Rheumatoid Arthritis.Biomolecules (2023) 13(7), 1106. DOI: 10.3390/biom13071106
Clerc, F., Novokmet, M., Dotz, V., Reiding, K.R., Haan, de N., Kammeijer, G.S.M., Dalebout, H., Bladergroen, M.R., Vukovic, F., Rapp, E. IBD-BIOM consortium, Targan, S.R., Barron, G., Manetti, N., Latiano, A., McGovern, D.P.B., Annese, V., Lauc, G., Wuhrer, M. Plasma N-Glycan Signatures of Inflammatory Bowel Diseases. Gastroenterology (2018) 155(3), 829–843. DOI: 10.1053/j.gastro.2018.05.030
Šimurina, M., ... Kammeijer, G.S.M.,... Wuhrer, M., Lauc, G. Glycosylation of immunoglobulin G associates with clinical features of inflammatory bowel diseases. Gastroenterology (2018) 154(5), 1320–1333.e10. DOI: 10.1053/j.gastro.2018.01.002
Plomp, R., Dekkers, G., Rombouts, Y., Visser, R., Koeleman, C.A.M., Kammeijer, G.S.M., Jansen, B.C., Rispens, T., Hensbergen, P.J., Vidarsson, G., Wuhrer, M. Hinge-Region O-Glycosylation of Human Immunoglobulin G3 (IgG3). Molecular and Cellular Proteomics (2015) 14(5), 1373–1384. DOI: 10.1074/mcp.M114.047381
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Wang, D., Madunić, K., Zhang, T, Lageveen-Kammeijer, G.S.M., Wuhrer, M. Profound Diversity of the N-Glycome from Microdissected Regions of Colorectal Cancer, Stroma, and Normal Colon Mucosa. Engineering (2022) 26, 32–43. DOI: 10.1016/j.eng.2022.08.016
Boyaval, F., Dalebout, H., Zeijl, R. van, Wang, W., Fariña-Sarasqueta, A., Lageveen-Kammeijer, G.S.M., Boonstra, J.J., McDonnell, L.A., Wuhrer, M., Morreau, H., Heijs, B. High-Mannose N-Glycans as Malignant Progression Markers in Early-Stage Colorectal Cancer. Cancers (2022) 14(6), 1552. DOI: 10.3390/cancers14061552
Sánchez López, E., Kammeijer, G.S.M., Crego, A.L., Luisa Marina, M., Ramautar, R., Peters, D.J.M., Mayboroda, O.A. Sheathless CE-MS Based Metabolic Profiling of Kidney Tissue Section Samples from a Mouse Model of Polycystic Kidney Disease. Scientific Reports (2019) 9, 806. DOI: 10.1038/s41598-018-37512-8
Cross-listed (see also Cancer Biomarkers & Oncology Applications):
Boyaval, F. … Cancers (2022) — Early-stage CRC progression markers
Guo, R-R, … Carbohydrate Polymers (2025) — Nnhuman N-Glycans in mammalian organs
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Wang, D., Madunić, K., Mayboroda, O.A., Lageveen-Kammeijer, G.S.M., Wuhrer, M. (Sialyl) Lewis Antigen Expression on Glycosphingolipids, N-, and O-Glycans in Colorectal Cancer Cell Lines is Linked to a Colon-Like Differentiation Program.Molecular and Cellular Proteomics (2024). DOI: 10.1016/j.mcpro.2024.100776
Mayboroda, O.A., Lageveen-Kammeijer, G.S.M., Wuhrer, M., Dolhain, R.J.E.M., An Integrated Glycosylation Signature of Rheumatoid Arthritis. Biomolecules (2023) 13(7), 1106. DOI: 10.3390/biom13071106
Blöchl, C., Wang, D., Mayboroda, O.A., Lageveen-Kammeijer, G.S.M., Wuhrer, M. Transcriptionally imprinted glycomic signatures of acute myeloid leukemia. Cell & Bioscience (2023) 13(1), 31. DOI: 10.1186/s13578-023-00981-0
Wang, D., Zhang, T, Madunić, K., de Waard, A.A., Blöchl, C., Mayboroda, O.A., Griffioen, M., Spaapen, R.M., Huber, C.G., Lageveen-Kammeijer, G.S.M., Wuhrer, M. Glycosphingolipid-Glycan Signatures of Acute Myeloid Leukemia Cell Lines Reflect Hematopoietic Differentiation.Journal of Proteome Research (2022) 21(4), 1029–1040. DOI: 10.1021/acs.jproteome.1c00911
Blöchl, C., Wang, D., Madunic, K., Lageveen-Kammeijer, G.S.M., Huber, C.G., Wuhrer, M., Zhang. Integrated N- and O-Glycomics of Acute Myeloid Leukemia (AML) Cell Lines. Cells (2021) 10(11), 3058. DOI: 10.3390/cells10113058
Cross-listed (see also Cancer Biomarkers & Oncology Applications):
AML publications listed above (same titles)
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Guo, R-R., Lageveen-Kammeijer, G.S.M., Wang, W., Dalebout, H., Zhang, W., Wuhrer, M., Liu, L., Heijs, B., Voglmeir, J. Analysis of Immunogenic Galactose-α-1,3-galactose-Containing N-Glycans in Beef, Mutton, and Pork Tenderloin by Combining Matrix-Assisted Laser Desorption/Ionization-Mass Spectroscopy and Capillary Electrophoresis Hyphenated with Mass Spectrometry via Electrospray Ionization. Journal of Agricultural and Food Chemistry (2023) 71(9), 4184–4192. DOI: 10.1021/acs.jafc.2c08067
Guo, R-R, Heijs, B., Wang, W., Wuhrer, M., Liu, L., Lageveen-Kammeijer, G.S.M.,* Voglmeir, J.,* Insight into distribution and composition of nonhuman N-Glycans in mammalian organs via MALDI-TOF and MALDI-MSI. Carbohydrate Polymers (2025) 351, 123065. DOI: 10.1016/j.carbpol.2024.123065
Beyond peer-reviewed papers, we contribute through patent applications, theses, book chapters, and technical/application notes that translate methods into practice and support community uptake. These outputs reflect both applied innovation and educational efforts in glyco(proteo)mics.
Other Ouputs
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Cobbaert, C. Kroeniger, K., Lageveen-Kammeijer, G.S.M., Nouta, J., Rolny, V., Ruhaak, R., Swiatek-de Lange, M., Tabares, G., Burgt, Y. van der, Wuhrer, M. Methods for management of prostate cancer. US20250116670A1 (18908787). (2025)
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Lageveen-Kammeijer, G.S.M. Unravelling the sugar-coating of prostate-specific antigen: Method development and its application to prostate cancer research. Thesis (2019). ISBN: 978-94-6380-249-9
Wang, W., Wang, W. and Lageveen-Kammeijer, G.S.M. (2022) CE-MS approaches for glyco(proteo)mic analysis. CE-MS for Proteomics and Metabolomics, Wiley-Verlag
Wang, W. and Lageveen-Kammeijer, G.S.M. (2022) High Sensitivity Glycoproteomic Analysis of Biological Samples by CE-ESI-MS. Capillary Electrophoresis-Mass Spectrometry: Methods and Protocols, Methods in Molecular Biology, Springer Nature (In Press)
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Protein Metrics, Intact Profiling and Visualization of Urinary Prostate-Specific Antigen (PSA) Profiles from Pooled and Individual Patient Samples (Application note)
Kammeijer, G.S.M., Kohler, I., Jansen, B.C., Hensbergen, P.J., Mayboroda, O.A., Falck, D., Lock, S., Wuhrer, M. Improved CESI-MS sensitivity and repeatability in Glycopeptide Analysis using a Dopant Enriched Nitrogen Gas.